TY  - GEN
T1  - RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans
AU  - Duan, Ye
AU  - Sun, Yongming
AU  - Ambros, Victor
DO  - 10.17912/micropub.biology.000312
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000312/
AB  - RNA-seq is widely used for the quantitative analysis of transcriptomes in the context of studies of gene expression and regulation (Mortazavi et al., 2008; Ozsolak and Milos, 2011; Wang et al., 2009). Generally, RNA-seq protocols employ poly(A) selection for mRNA enrichment. However, poly(A) based enrichment is subject to potential bias depending on the poly(A) status of various mRNAs, which could be particularly undesirable in the context of studying post-transcriptional gene regulatory mechanisms, such as miRNA repression (Wu et al., 2006). Therefore, ribosomal RNA (rRNA) depletion is a desirable alternative strategy to enrich for mRNA sequences in RNA-seq sample preparation (Zhao et al., 2014). However, currently available rRNA depletion toolkits were designed for either mammals or bacteria, and hence do not offer an efficient option for rRNA depletion of RNA samples from certain experimental organisms, such as C. elegans.
PY  - 2020
JO  - microPublication Biology
ER  -