TY  - GEN
T1  - C. elegans CYLC-2 localizes to sperm
AU  - Krauchunas, Amber R
AU  - Werner, Michael
AU  - Britt, Nicholas
AU  - Chen, Dawn S
AU  - Maddox, Amy S
AU  - Singson, Andrew
DO  - 10.17912/micropub.biology.000314
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000314/
AB  - The gene products of C. elegans ORFs Y59E9AL.6 and C41G7.6 were assigned the names CYLC-1 and CYLC-2, respectively, due to their homology to human cylicins (Lacroix et al.. 2016). In mammals, expression of cylicin I and cylicin II is testis-specific and immunohistochemistry shows the proteins localize to the cytoskeletal calyx of the sperm head (Hess et al. 1993; Hess et al. 1995; Rousseaux-Prèvost et al. 2003). To determine the localization of C. elegans CYLC-1 and CYLC-2 we used CRISPR/Cas9 to endogenously tag each protein with mNeonGreen (mNG). We find that CYLC-2::mNG localizes to sperm in both hermaphrodites and males (Figure 1A-F). Examination of spermatids dissected from males shows CYLC-2::mNG concentrated in puncta (Figure 1F). Based on their size and position in the spermatids, we predict that these puncta correspond to the membranous organelles (MOs). However, further studies will be necessary to confirm whether CYLC-2 is concentrated in the MOs of spermatids and to determine if any changes in subcellular localization take place upon sperm activation.
PY  - 2020
JO  - microPublication Biology
ER  -