TY  - GEN
T1  - A Plate Based Assay for Determination of the Median Lethal Dose of 1-Hydroxyphenazine in Caenorhabditis elegans
AU  - Asif, Muhammad Zaka
AU  - Van der Gaag, Victoria L.
AU  - Guo, Jane
AU  - Nocilla, Kelsey A.
AU  - Muzio, Cole J.
AU  - Edison, Arthur S.
DO  - 10.17912/micropub.biology.000352
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000352/
AB  - 1-Hydroxyphenazine (1-HP) is a small molecule produced by Pseudomonas aeruginosa, a bacterium that is used for pathogenesis models in C. elegans (Cezairliyan et al., 2013; Mahajan-Miklos, Tan, Rahme, & Ausubel, 1999). 1-HP is an especially interesting toxin to study as it has been shown to interact with human cells causing ciliary-slowing associated with dyskinesia and ciliostasis (Wilson et al., 1987). Prior research in our lab has shown that this molecule is toxic to C. elegans, with an LD50 between 150 and 200 μM, but C. elegans can glycosylate 1-HP, which detoxifies the molecule (Stupp et al., 2013).
PY  - 2021
JO  - microPublication Biology
ER  -