TY  - GEN
T1  - A genome-wide screen in Saccharomyces cerevisiae identifies Tannic Acid-sensitive mutants
AU  - Pilc, Emily M
AU  - Ganesh, Shriie
AU  - Kerscher, Oliver
DO  - 10.17912/micropub.biology.000358
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000358/
AB  - We conducted a genome-wide chemical genetics screen of the yeast MATa knockout collection to identify genes that are required for survival on sub-lethal levels of the polyphenol Tannic Acid (TA) (see Fig 1A and reagents). TA, a plant-derived polymeric form of gallic acid connected to a central glucose core, is known to bind and precipitate proteins (Hagerman and Klucher 1986), chelate iron (ferric ions, Fe3), and form TA-Fe3 films on cells, including yeast (Lee et al. 2020). Addition of TA to cultured prostate cancer cells is reported to induce ER stress, increase markers of the unfolded protein response (UPR), and inhibit lipid metabolism (Nagesh et al. 2020). TA has also been reported to act as a potent but non-toxic SUMO E1 inhibitor in several cell lines (Suzawa et al. 2015). How TA induces these effects remains unknown and, therefore, the genome-wide TA-sensitivity screen may provide insights into underlying genetic pathways involved.
PY  - 2021
JO  - microPublication Biology
ER  -