TY  - GEN
T1  - Mutations in the Physcomitrium patens gene encoding Aminodeoxychorismate Synthase confer auxotrophic phenotypes
AU  - Prigge, Michael J
AU  - Wang, Yingluo
AU  - Estelle, Mark
DO  - 10.17912/micropub.biology.000364
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000364/
AB  - The haploid-dominant life cycle of mosses poses a challenge to genetically mapping infertile mutants. Recently, a method to circumvent these difficulties was developed that uses protoplast fusion of mutant and wild-type protoplasts to produce fertile somatic hybrids that produce segregating sporelings upon selfing (Moody et al. 2018). All known methods for producing somatic hybrids require the parent strains to either have complementing auxotrophic mutations or distinct antibiotic resistances (Grimsley et al. 1977; Cove et al. 2009b). Here we describe a strain that could be used as a universal fusion partner for mutants that contain neither type of marker, one that combines recessive auxotrophy with dominant antibiotic resistance.
PY  - 2021
JO  - microPublication Biology
ER  -