TY  - GEN
T1  - Genetic Mapping of a new Hippo allele, HpoN.1.2, in Drosophila melanogaster
AU  - Siders, Jamie L
AU  - Bieser, Kayla L
AU  - Hamill, Danielle R
AU  - Acosta, Erika C
AU  - Alexander, Olivia K
AU  - Ali, Humza I
AU  - Anderson, Micah J
AU  - Arrasmith, Hayden R
AU  - Azam, Mustafa
AU  - Beeman, Nikki J
AU  - Beydoun, Hassan
AU  - Bishop, Lauren J
AU  - Blair, Morgan D
AU  - Bletch, Brianna
AU  - Bline, Heather R
AU  - Brown, Jennifer C
AU  - Burns, Kelly M
AU  - Calagua, Karina C
AU  - Chafin, Lexie
AU  - Christy, William AH
AU  - Ciamacco, Carlyn
AU  - Cizauskas, Hannah
AU  - Colwell, Caitlyn M
AU  - Courtright, Abigail R
AU  - Diaz Alavez, Lucero
AU  - Ecret, Rayne IS
AU  - Edriss, Fatima
AU  - Ellerbrock, Taylor G
AU  - Ellis, Madison M
AU  - Extine, Erica M
AU  - Feldman, Eric
AU  - Fickenworth, Luke J
AU  - Goeller, Caroline M
AU  - Grogg, Alexis S
AU  - Hernandez, Yailine
AU  - Hershner, Abigail
AU  - Jauss, Megan M
AU  - Jimenez Garcia, Leyre
AU  - Franks, Katey E
AU  - Kazubski, Ethan T
AU  - Landis, Emily R
AU  - Langub, Jon
AU  - Lassek, Tia N
AU  - Le, Triet C
AU  - Lee, Julia M
AU  - Levine, Daniel P
AU  - Lightfoot, Phoebe J
AU  - Love, Natasha
AU  - Maalhagh-Fard, Ali
AU  - Maguire, Colin
AU  - McGinnis, Brynna E
AU  - Mehta, Bhargavi V
AU  - Melendrez, Veronica
AU  - Mena, Zimri E
AU  - Mendell, Seth
AU  - Montiel-Garcia, Petra
AU  - Murry, Autumn S
AU  - Newland, Riley A
AU  - Nobles, Ryan M
AU  - Patel, Neha
AU  - Patil, Yashodhara
AU  - Pfister, Cassidy L
AU  - Ramage, Victoria
AU  - Ray, Mya R
AU  - Rodrigues, Joseph
AU  - Rodriquez, Victoria C
AU  - Romero, Yara
AU  - Scott, Alexandra M
AU  - Shaba, Nicholas
AU  - Sieg, Samantha
AU  - Silva, Kayla
AU  - Singh, Sahiba
AU  - Spargo, Aleksandria J
AU  - Spitnale, Savanna J
AU  - Sweeden, Nicole
AU  - Tague, Logan
AU  - Tavernini, Breanna M
AU  - Tran, Kathleen
AU  - Tungol, Liselle
AU  - Vestal, Kylie A
AU  - Wetherbee, Amber
AU  - Wright, Kayla M
AU  - Yeager, Anthony T
AU  - Zahid, Rehab
AU  - Kagey, Jacob D
DO  - 10.17912/micropub.biology.000383
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000383/
AB  - In order to identify conditional regulators of cell growth and tumorigenesis, an EMS-based genetic screen was conducted in Drosophila melanogaster utilizing the Flp/FRT system in an apoptotic null background (Akdemir et al., 2006). A fly line possessing the FRT42D, Dark82 chromosome and containing a mini-white cassette (w+mC) was used for EMS mutagenesis. Subsequent matings to FRT42D; Ey-Flp flies facilitated phenotypic screening in eye tissue. Phenotypes observed in the screen included alterations in mosaicism (red > white pigmentation pattern), eye and/or antennal overgrowth, defects in patterning, and pupal lethality (Kagey et al. 2012). One of the pupal lethal mutants identified in this screen, N.1.2, is discussed here. Genetic crosses between FRT42D, Dark82, N.1.2 X FRT42D; Ey-Flp resulted in near complete pupal lethality (~ 90%) due to dramatic tissue overgrowth of the eye, antennae, and inter-ocular space (Figure 1B compared to 1A). Due to the pupal lethality associated with the eye tissue overgrowth, flies were dissected from late pupal stages in order to visualize the phenotype. Imaging following dissection shows that the mutant eyes are comprised of mostly mutant (pigmented) tissue that protrudes from the eye cavity creating tissue folding (Figure 1B). Control FRT42D, Dark82 flies exhibited a balance in the red:white ratio and no eye overgrowth (Figure 1A). For direct comparison to N.1.2, the FRT42D, Dark82 mosaic eye was also imaged at the late pupal stage. Eyes were imaged under 70% ethanol on an AM Scope digital camera at 40x using a LED light ring.
PY  - 2021
JO  - microPublication Biology
ER  -