TY  - GEN
T1  - Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
AU  - Gallegos, Maria
AU  - Hermes, Alex
AU  - Patra, Debjani
DO  - 10.17912/micropub.biology.000389
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000389/
AB  - UNC-11 is an ortholog of mammalian PICALM (phosphatidylinositol binding clathrin assembly protein) and its neuron-specific paralog, AP180 (Maritzen et al. 2012). PICALM facilitates recycling of Vesicle Associated Membrane Proteins (VAMPs) from the plasma membrane (Miller et al. 2011, Sahlender et al. 2013 and Miller et al. 2015) and efficient clathrin-coated endocytosis (Huang et al. 2004, Miller et al. 2015 and Ishikawa et al. 2014). unc-11 function in C. elegans is likely conserved as SNB-2 (VAMP2) and the glutamate receptor, GLR-1, both accumulate at the plasma membrane in unc-11(e47) null mutants (Nonet et al. 1999, Burbea et al. 2002). Mutant phenotypes outside the worm nervous system have not been described.
PY  - 2021
JO  - microPublication Biology
ER  -