TY  - GEN
T1  - Optimizing expression of a single copy transgene in C. elegans
AU  - Dour, Scott
AU  - Nonet, Michael
DO  - 10.17912/micropub.biology.000394
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000394/
AB  - Several methods are currently available to create single copy C. elegans transgenic animals that express recombinant proteins under the control of specific promoters. Unfortunately, in many cases these transgenic animals express the engineered tools at levels too low to be practically used in routine experiments. We examined the effects of modifications to the trans-splicing sequence, the Kozak ribosomal binding site that promotes translational initiation (Kozak 1987), the N-terminal amino acid controlling N-end rule-mediated protein stability, and the 3′ UTR sequences which regulate message stability on steady state GFP levels in a set of single copy insertions at the same position. Our results document that modifying each of the three components can influence expression levels.
PY  - 2021
JO  - microPublication Biology
ER  -