TY  - GEN
T1  - Swimming exercise reduces native ⍺-synuclein protein species in a transgenic C. elegans model of Parkinson’s disease.
AU  - Schmidt, Minna Y.
AU  - Chamoli, Manish
AU  - Lithgow, Gordon J.
AU  - Andersen, Julie K.
DO  - 10.17912/micropub.biology.000413
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000413/
AB  - Parkinson’s disease (PD) patients have been shown to benefit greatly from intense physical activity (Schenkman et al. 2018). Recent studies have demonstrated that exercise causes changes in the levels of ⍺-synuclein aggregate species, a hallmark of PD, in different mammalian animal models (Koo and Cho 2017; Shin et al. 2017; Zhou et al. 2017; Minakaki et al. 2019). However, questions still remain about how exercise affects specifically native ⍺-synuclein protein species directly after the cessation of exercise and the longer-term downstream effects which exercise may have on organismal health. It was recently discovered that periods of thrashing in liquid solution, otherwise called swimming exercise, in C. elegans worms, induces many mechanisms invoked during mammalian exercise (Laranjeiro et al. 2017). This has provided an avenue for studying exercise conditions in various C. elegans models of neurodegeneration (Laranjeiro et al. 2019). In order to study the effect of exercise on native human ⍺-synuclein protein species, we utilized the NL5901- pkIs2386 worm model of Parkinson’s which contains human ⍺-synuclein tagged to a yellow fluorescent protein (YFP) in the muscle cells (van Ham et al. 2008). We performed tissue analysis via Blue Native (BN) page westerns and confocal microscopy. In addition, because pharyngeal pumping is decreased while worms are swimming, we controlled for this effect by exposing worms in parallel to a period of food restriction (FR) conditions (Vidal-Gadea et al. 2012). We also performed thrashing assays to assess longer term downstream behavioral effects on the animals after either exercise or food restriction conditions.
PY  - 2021
JO  - microPublication Biology
ER  -