TY  - GEN
T1  - The conditional growth suppressor E7.25d.7 is an allele of the sterile-20 kinase misshapen
AU  - Kiely, Evan
AU  - Gilbert-Ross, Melissa
DO  - 10.17912/micropub.biology.000424
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000424/
AB  - The lethal allele E7.25d.7 was isolated in a previously described FLP/FRT screen for conditional growth suppressors on chromosome 3L (Gilbert et al., 2011). Briefly, male w1118;; Df(3L)H99,FRT80B/TM6B flies were fed 25mM EMS and mated en mass to eyFLP;;ubi-GFP, FRT80B virgin females to screen a total of ~20,000 chromosome arms. Although most alleles from the screen were selected based on overgrowth of mutant white tissue relative to red wild-type twin spots, E7.25d.7 retained a mostly 50:50 white:red ratio, and apparent cell shape and bristle defects (Fig. 1A). To test whether our E725d.7 allele could synergize with Df(3L)H99 to override organ size controls, we crossed H99,E7.25d.7,FRT80B/TM6B to eyFLP;; RpL141,FRT80B/TM6B, a Minute allele (Saeboe-Larssen et al., 1997), thus allowing us to create entire heads that were mutant for E725d.7 and lacked developmental cell death. Compared to Df(3L)H99,FRT80B/FRT80B,RpL141 control heads, H99,E7.25d.7,FRT80B/FRT80B,RpL141 heads were larger and malformed, contained increased numbers of thick bristles, evidence of necrosis, and tumor-like morphological abnormalities (Fig. 1B). These phenotypes had different levels of expressivity, with females consistently exhibiting the most severe phenotypes.
PY  - 2021
JO  - microPublication Biology
ER  -