TY  - GEN
T1  - Dynein and MEL-28 contribute in parallel to oogenic maturity in C. elegans
AU  - Gandhi, Jay
AU  - Crosio, Giulia
AU  - Fernandez, Anita G.
DO  - 10.17912/micropub.biology.000421
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000421/
AB  - Most cellular processes require the coordinate activities of multiple gene products. These collaborations vary with different contexts. mel-28 encodes a conserved multi-functional nucleoporin required for nuclear envelope integrity, the post-mitotic rebuilding of the nuclear pore, and the proper segregation of chromosomes during mitosis (Galy et al. 2006; Fernandez and Piano 2006). Loss of mel-28 results in strict maternal-effect embryonic lethality. Thus homozygous mutant embryos produced from heterozygous mothers show typical wild-type development until they become adults and produce normal-sized broods of inviable embryos. This suggests that mel-28 is required for embryonic development but is dispensable for post-embryonic development and adult processes such as fertility. To identify genes that function collaboratively with mel-28, we did an RNAi screen to find genes that cause fertility defects in mel-28 mutant animals but not wild-type animals (Fernandez et al. 2014). Among the genes identified were those encoding components of the minus-end-directed microtubule motor dynein.
PY  - 2021
JO  - microPublication Biology
ER  -