TY  - GEN
T1  - The primordial germ line is refractory to perturbations of actomyosin regulator function in C. elegans L1 larvae
AU  - Bauer, Jack
AU  - Lacroix, Léa
AU  - Labbé, Jean-Claude
DO  - 10.17912/micropub.biology.000432
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000432/
AB  - To perturb actomyosin function in the primordial germ line, we first monitored germ line organization in L1-stage animals bearing temperature-sensitive (ts) alleles in genes encoding actomyosin regulators and that were reported to interfere with cytokinesis during embryogenesis (Davies et al. 2014). Previous work demonstrated that the initial stages of germline expansion occur normally in cyk-4(ts) and zen-4(ts) animals raised at restrictive temperature from the L1 stage (Lee et al. 2018). We found that primordial germ line organization in cyk-1(ts), nmy-2(ts), cyk-4(ts) or zen-4(ts) L1 larvae maintained at restrictive temperature for 12h was no different than control (Figure 1A-B). Furthermore, the first primordial germ cell (PGC) division occurred normally upon feeding these animals at restrictive temperature with typical bacterial food (E. coli OP50). As noted previously (Lee et al. 2018), germ line disorganization and sterility were observed in all cases when animals reached adulthood (Figure 1B).
PY  - 2021
JO  - microPublication Biology
ER  -