TY  - GEN
T1  - Direct comparisons of bisulfite pyrosequencing versus targeted bisulfite sequencing
AU  - King, Dillon E.
AU  - Sparling, A. Clare
AU  - Joglekar, Rashmi
AU  - Meyer, Joel N.
AU  - Murphy, Susan K.
DO  - 10.17912/micropub.biology.000444
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000444/
AB  - DNA methylation typically refers to the covalent bonding of a methyl group (-CH3) to the 5’ carbon of a cytosine residue. These methylated cytosines, referred to as 5-mC, most often precede guanines in the DNA sequence and are referred to as CpG methylation (Rakyan et al. 2011). DNA methylation is a critical component of the regulatory mechanisms that assure proper genome function. It plays an important role in embryonic development, X chromosome inactivation, genomic imprinting, and defense against the activity and transposition of repeat elements. Methylation is catalyzed by DNA methyltransferase enzymes (DNMTs) (Jeltsch 2006; Denis et al. 2011). Dysregulation of DNA methylation via mutations in DMNTs, disease processes or from environmental exposures is associated with neurodevelopmental disorders, chronic disease and cancers (Greenberg and Bourc’his 2019).
PY  - 2021
JO  - microPublication Biology
ER  -