TY  - GEN
T1  - Live-cell imaging reveals square shape spindles and long mitosis duration in the silkworm holocentric cells
AU  - Vanpoperinghe, Lucien
AU  - Carlier-Grynkorn, Frederique
AU  - Cornilleau, Gaetan
AU  - Kusakabe, Takahiro
AU  - Drinnenberg, Ines A
AU  - Tran, Phong T
DO  - 10.17912/micropub.biology.000441
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000441/
AB  - Human hTERT-RPE1 cells represent wild-type monocentric cells as they were immortalized, via telomerase expression, from normal somatic retinal pigment epithelium cells (Jiang et al. 1999). Silkworm BmN4-SID1 cells represent wild-type holocentric cells as they were spontaneously immortalized from somatic ovary cells, and modified with the SID1 gene from C. elegans for RNAi-mediated depletion (Mon et al. 2012). Interestingly, cancer such as leukemia and lymphoma often exhibits dicentric or tricentric chromosomes (Herry et al. 2007; M’Kacher et al. 2020), a condition reminiscent of holocentric cells. Further, monocentric cells induced to be dicentric showed transformed cancerous properties (Gascoigne and Cheeseman 2013). We seek to understand the differences in spindle assembly dynamics and chromosome segregation in wild-type monocentric RPE1 and holocentric BmN4 cells, to potentially reveal mechanisms of how di- or tricentric cancer cells divide.
PY  - 2021
JO  - microPublication Biology
ER  -