TY  - GEN
T1  - Transgenic reporter analysis of ChIP-Seq-defined enhancers identifies novel target genes for the terminal selector UNC-3/Collier/Ebf
AU  - Li, Yinan
AU  - Kratsios, Paschalis
DO  - 10.17912/micropub.biology.000453
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000453/
AB  - The role of UNC-3 in establishing and maintaining the cell identity of cholinergic motor neurons (MNs) has been investigated in several studies (Feng et al., 2020; Kratsios et al., 2011; Li et al., 2020). Through a candidate approach, more than 50 terminal identity genes have been identified genetically as downstream targets of UNC-3. These genes encode proteins essential for the proper functions of cholinergic MNs and include acetylcholine biosynthesis components, ion channels, neurotransmitter receptors, and neuropeptides (Kratsios et al., 2011). To test whether UNC-3 directly controls the expression of these genes, we recently performed Chromatin Immunoprecipitation followed by Sequencing (ChIP-Seq) analysis for UNC-3. Indeed, UNC-3 directly binds to the cis-regulatory region of these ~50 known target genes via a consensus DNA binding site, termed COE motif (Li et al., 2020). This suggests that activating terminal identity genes through physical binding to their loci is a key strategy employed by UNC-3 to regulate cholinergic MN identity.
PY  - 2021
JO  - microPublication Biology
ER  -