TY  - GEN
T1  - Identification and in silico analysis of the origin recognition complex in the human fungal pathogen Candida albicans
AU  - Padmanabhan, Sreedevi
AU  - Sanyal, Kaustuv
AU  - Dubey, Dharanidhar
DO  - 10.17912/micropub.biology.000465
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000465/
AB  - DNA replication in eukaryotes is initiated by the orchestrated assembly and association of initiator proteins on the replication origins. The hunt for initiator proteins in higher eukaryotes picked up pace after the discovery of the Origin Recognition Complex (ORC) comprising of six protein subunits of the ORC1-6 complex in budding yeast (Bell and Stillman, 1992). In Candida albicans, a human fungal pathogen, the ORC (CaORC1-6) and their associated proteins were identified by a BLAST analysis using the S. cerevisiae proteins as the query sequences in the Candida Genome Database (CGD). The phylogenetic analysis suggests that in spite of limited amino acid sequence similarity with their counterparts in other organisms, the CaORC proteins share most of the functional domains with them. Interestingly, the amino acid sequences of CaORC1, 2 and 4 share higher degree of similarities than CaORC3, 5 and 6 to those of S. cerevisiae. CaORC1, 4 and 5 tend to be homologous to the mammalian counterparts. Although, in general, the CaORC proteins show limited sequence similarities with their counterparts in various species, CaORC1, 2 and 6 show maximum similarities to their S. cerevisiae counterparts while CaORC3, 4 and 5 appear to be more similar to those of mammals which is evident from the phylogenetic map (Figure 1A, Extended Data Tables 1-3).
PY  - 2021
JO  - microPublication Biology
ER  -