TY  - GEN
T1  - Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
AU  - Nonet, Michael
DO  - 10.17912/micropub.biology.000438
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000438/
AB  - Several bipartite systems have been described for use with C. elegans (Wei et al., 2012; Wang et al., 2017; Wang et al., 2018; Mao et al., 2019; Nonet, 2020). However, little manipulation of the drivers or reporters has been performed to optimize them for C. elegans. Mao. et. al. 2019 demonstrated that the QF activation domain is a more potent activator than either VP64 or the hybrid VP64-p65-Rta tripartite activatorVPR. Here, I describe modifications of linker region between the activation domains of both GAL4SK and TetR drivers that increase the activity of these reporters. Although these studies are not comprehensive, I opted to describe them herein because the modification of the GAL4SK driver increases the activity of this driver substantially such that it is now on par with the LexA, TetR and QF2 drivers.
PY  - 2021
JO  - microPublication Biology
ER  -