TY  - GEN
T1  - Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans
AU  - Huang, George
AU  - de Jesus, Bailey
AU  - Koh, Alex
AU  - Blanco, Sara
AU  - Rettmann, Aubrie
AU  - DeMott, Ella
AU  - Sylvester, Melynda
AU  - Ren, Cassie
AU  - Meng, Carrie
AU  - Waterland, Skye
AU  - Rhodes, Anita
AU  - Alicea, Persephone
AU  - Flynn, Abbey
AU  - Dickinson, Daniel J
AU  - Doonan, Ryan
DO  - 10.17912/micropub.biology.000460
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000460/
AB  - The self-excising cassette (SEC) knock-in approach uses hygromycin selection and a visible roller phenotype to identify knock-ins, followed by a heat-shock induced excision of these visible markers to yield a seamless insertion of a fluorescent protein into the genome (Dickinson et al. 2015). Compared to protocols that utilize Cas9 protein and linear DNA repair templates (Paix et al. 2015; Dokshin et al. 2018; Ghanta and Mello 2020), the plasmid-based SEC approach employs a simpler screening strategy but requires more worms to be injected (Dickinson and Goldstein 2016).
PY  - 2021
JO  - microPublication Biology
ER  -