TY  - GEN
T1  - Endogenous expression and localization of HIS-72::mTurquoise2 in C. elegans
AU  - Sloan, Dillon E.
AU  - Bembenek, Joshua N.
DO  - 10.17912/micropub.biology.000471
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000471/
AB  - In fixed samples, chromatin is most often visualized by using a fluorescent DNA-binding dye like DAPI, which emits blue light (Kapuscinski 1995). In live fluorescence microscopy, however, a fluorescently tagged histone is often used instead. Histones form nucleosomes to package chromatin, and are involved in the organization of the genome (Chen et al. 2021). Most frequently, histones are fused to red or green fluorescent proteins, though the use of a blue or cyan fluorescent protein instead would allow for three (or more) channel imaging (Kanda et al. 1998, Das et al. 2003, Ooi et al. 2006).
PY  - 2021
JO  - microPublication Biology
ER  -