TY  - GEN
T1  - Using the Drosophila Transcriptional Reporter of Intracellular Calcium (TRIC) to examine lasting ethanol-induced changes in neuroexcitability
AU  - Merriman, Kahlan
AU  - Petruccelli, Emily
DO  - 10.17912/micropub.biology.000477
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000477/
AB  - Alcohol Use Disorder (AUD) is associated with the disruption of various neurotransmitter systems including dopaminergic reward pathways (Banerjee 2014; Abrahao et al. 2017). For example, acute alcohol in low or moderate doses can potentiate or inhibit neuronal activity of distinct brain regions in the human central nervous system (Harrison et al. 2017). Similar observations have also been made in rodent AUD models, although different experimental approaches have highlighted the unresolved complexity of ethanol’s impact on neuroexcitability. For instance, low-ethanol-drinking mice showed increased ventral tegmental area (VTA) dopamine neuron firing, but surprisingly, high-ethanol-drinking animals displayed similar firing to that of naïve animals (Juarez et al. 2017). Drosophila melanogaster (flies) have also become an established model for the study of AUD, offering unbiased high-throughput screening, detailed behavioral analyses, and a platform for creating novel genetic tools (Devineni et al. 2011; Kaun et al. 2012). Intoxicated flies display acute ethanol-induced hyperactivity, loss of postural control, sedation, and develop both rapid and chronic tolerance. Furthermore, flies can make addiction-like reward memories of ethanol following repeated exposures (Kaun et al. 2011). To date, it remains unclear whether the long-term neuroexcitability of particular adult fly neurons are affected by acute or repeated ethanol exposure.
PY  - 2021
JO  - microPublication Biology
ER  -