TY  - GEN
T1  - SGS1-SuOff rescues the mild methylmethane sulfonate sensitivity of srs2Δ cells in Saccharomyces cerevisiae
AU  - Herce-Hagiwara, Belen
AU  - Thu, Yee Mon
DO  - 10.17912/micropub.biology.000480
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000480/
AB  - The RecQ helicases play a crucial role in the maintenance of genome stability. The importance of these helicases is most apparent in genetic diseases associated with RecQ helicases. In human, defects in BLM, RECQL4 or WRN result in Bloom’s syndrome, Rothmund–Thomson syndrome and Werner’s syndrome, respectively (Ellis et al. 1995; Yu et al. 1996; Kitao et al. 1999). A common characteristic of all these syndromes is a predisposition to genome stability and cancer (Croteau et al. 2014). In Saccharomyces cerevisiae, Sgs1p is a RecQ helicase homolog. Sgs1p functions as part of the STR complex (Sgs1p-Top3p-Rmi1p complex) and regulates recombination to prevent illegitimate structures (Croteau et al. 2014). For instance, cells deficient in SGS1 accumulated Rad51p-dependent aberrant DNA structures at replication forks when cells were treated with methylmethane sulfonate (MMS) (Liberi et al. 2005). How Sgs1p regulates recombination, in part, depends on sumoylation, a process in which a SUMO (small ubiquitin-like modifier) peptide is covalently conjugated to target proteins. In response to DNA damage, Sgs1p becomes sumoylated (Branzei et al. 2006; Bermúdez-López et al. 2016; Bonner et al.. 2016). Sumoylation of Sgs1p is mediated by an E3 SUMO ligase, Mms21p and this modification supports the ability of Sgs1p to resolve aberrant DNA structures at replication forks (Branzei et al. 2006; Bermúdez-López et al. 2016; Bonner et al. 2016).
PY  - 2021
JO  - microPublication Biology
ER  -