TY  - GEN
T1  - Imaging of dynamic actin remodeling reveals distinct behaviors of head and trunk mesoderm in gastrulating Xenopus laevis
AU  - Komatsu, Valerie
AU  - Doddihal, Viraj
AU  - Chang, Chenbei
DO  - 10.17912/micropub.biology.000483
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000483/
AB  - To visualize actin dynamics, cell shape changes, and cell movements during Xenopus laevis gastrulation, we injected mRNAs encoding LifeAct-GFP (Shindo et al., 2019) and histone H2B-RFP (Butler and Wallingford, 2018) into early Xenopus embryos. At the mid-gastrula stages, we dissected the dorsal tissues around the anterior region of the post-involuted bottle cells (Fig. 1A). We then placed the explants with the endodermal side down on fibronectin-coated glass-bottom dishes. Time-lapse microscopy was performed on an inverted confocal microscope. We captured the movements of the head and trunk mesodermal cells, visualizing distinct behaviors in the two regions (Fig. 1A). Below, we describe in more detail a movie that documented head mesoderm migration and notochord segregation in these regions. Cell movements were recorded in an explant for 8 hours with a capture interval of 4 minutes (Extended Data Movie 1).
PY  - 2021
JO  - microPublication Biology
ER  -