TY  - GEN
T1  - Cryopreservation of dehydrated Caenorhabditis elegans with multiple recoveries using a granular medium
AU  - Hayden, Julia
AU  - Fang-Yen, Christopher
DO  - 10.17912/micropub.biology.000488
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000488/
AB  - One advantage of C. elegans as an experimental model is its ability to be indefinitely preserved at ultracold temperatures. Two solutions are commonly used for cryopreservation of C. elegans. The liquid freezing buffer (Brenner 1974) contains glycerol as cryoprotectant. Since worms frozen with this solution settle to the bottom of the cryotube, recovering them requires thawing the entire tube. As a result, only one recovery is possible per tube. In the soft agar method developed by Leon Avery (Stiernagle 2006), the freezing solution contains agar in addition to glycerol. The agar gel keeps worms suspended throughout the tube during freezing. This allows a small portion of the tube to be scooped out for recovery, with the remainder returned to the freezer without thawing. Worms can typically be recovered 3-4 times from 1 mL of soft agar (Stiernagle 2006). The ability to perform several recoveries per tube reduces the need to prepare and store multiple tubes for cryopreservation.
PY  - 2021
JO  - microPublication Biology
ER  -