TY  - GEN
T1  - Microscopy analysis of the smallest subunit of the RPA complex, Rfa3p, prompts consideration of how RPA subunits gather at single-stranded DNA sites
AU  - Ramonatxo, Agnès
AU  - Moriel-Carretero, María
DO  - 10.17912/micropub.biology.000493
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000493/
AB  - The conserved, heterotrimeric RPA complex is involved in genome stability maintenance at multiple levels. Given its affinity for single-stranded DNA, which it protects from unwanted degradation, this complex is implicated in DNA repair, replication, and recombination (Maréchal and Zou 2015). In Saccharomyces cerevisiae, its three subunits are called Rfa1p, Rfa2p and Rfa3p. The biggest ones, Rfa1p and Rfa2p, contact DNA directly and are also responsible for the interaction with most protein partners (for a comprehensive curation see (Maréchal and Zou 2015)). As such, their fluorophore-tagged versions have recurrently been used in microscopy studies to monitor the implication of the RPA complex in the aforementioned processes ((Lisby et al. 2004; Ivanova et al. 2020; Wong et al. 2020), to cite some examples). Rfa3p is the smallest subunit, with a theoretical molecular weight of 14 kDa. Its position within the 3D-structure (Yates et al. 2018) as well as other studies (Bochkareva et al. 2002) let think that Rfa3p fulfills an exclusively structural role, probably explaining why it has attracted so little interest for microscopy approaches. Only one work, to our knowledge, has simultaneously assessed the subcellular distribution of the C-terminally tagged versions of the three subunits, concluding they all mainly share a nuclear localization (Belanger et al. 2011). Yet, that work did not assess their potentially similar (or different) response to replication challenges or to DNA damage.
PY  - 2021
JO  - microPublication Biology
ER  -