TY  - GEN
T1  - A new gene on C. elegans chromosome V
AU  - Iyengar, Anusha
AU  - Diamantakis, Stavros
AU  - Norris, Adam
DO  - 10.17912/micropub.biology.000496
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000496/
AB  - In the decades since the C. elegans genome sequence was completed (C. elegans Sequencing Consortium, 1998), ongoing curation efforts continue to further improve and annotate the genome (Harris et al., 2020). In the course of searching for alternatively-spliced genes using RNA-seq data, we happened upon an alternative splicing event in a region of the C. elegans genome with no associated gene or feature annotation. Upon closer inspection, we identified RNA-seq support for an unannotated four-exon gene, with the third exon being alternatively spliced (Fig 1A). The putative coding sequences of this gene encode proteins of either 86 or 122 amino acids, depending on the splicing choice. The longer isoform (exon three included) is the major isoform according to our RNA-seq data (Fig 1B), and this isoform encodes four predicted transmembrane domains (Fig 1C). RT-PCR of wild-type L4 stage worms further confirms that the gene is expressed at detectable levels, and that exon three is alternatively spliced (Fig 1D). We have submitted this information to WormBase, and the new gene is set to appear as WBGene00306123 (transcripts Y97E10C.2a and Y97E10C.2b) in release WS283.
PY  - 2021
JO  - microPublication Biology
ER  -