TY  - GEN
T1  - The proline-rich domain of MML-1 is biologically important but not required for localization to target promoters
AU  - Ceballos, Ainhoa
AU  - Esse, Ruben
AU  - Grishok, Alla
DO  - 10.17912/micropub.biology.000498
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000498/
AB  - The predominant and ubiquitously expressed C. elegans histone H3 lysine 79 methyltransferase DOT-1.1 and its interacting partner, ZFP-1, play important roles in negatively modulating widely and highly expressed genes through promoter binding (Cecere et al. 2013) and in creating permissive environment for expression of developmentally regulated genes through enhancers (Esse et al. 2019; Esse and Grishok 2020). However, the mechanistic aspects of the ZFP-1/DOT-1.1 complex function are not clear. This study was motivated by the observation that genome-wide promoter-associated ZFP-1 and DOT-1.1 chromatin localization peaks (Mansisidor et al. 2011; Cecere et al. 2013) overlap with those of the O-GlcNAc modification on chromatin observed in wild type C. elegans (Love et al. 2010) (Figure 1A). Notably, the identities of the O-GlcNAc-modified proteins contributing to the O-GlcNAc ChIP-chip signals at the select promoters remain unknown. We chose to investigate the C. elegans basic helix- loop-helix leucine-zipper (bHLH/ZIP) transcription factor MML-1 (Myc and Mondo-like 1) (Pickett et al. 2007; Grove et al. 2009) as a candidate O-GlcNAc-modified protein, because both c-Myc (Chou et al. 1995) and the Mondo B protein (i.e., the Carbohydrate Response Element Binding Protein (ChREBP)) (Guinez et al. 2011) carry O-GlcNAc modification. MML-1 is a 1009 amino acid protein with multiple conserved domains, including the N-terminal Mondo Conserved Regions (I-V) (Pickett et al. 2007), which form a glucose sensing module in ChREBP (Abdul-Wahed et al. 2017), a proline-rich region, similar to that in ChREBP (Ortega-Prieto and Postic 2019), and a C-terminal DNA-binding and MXL-2 heterodimerization region called bHLH/ZIP (Pickett et al. 2007; Grove et al. 2009) (Figure 1B).
PY  - 2021
JO  - microPublication Biology
ER  -