TY  - GEN
T1  - An analog-sensitive allele of Aurora kinase B is lethal in mouse
AU  - Vazquez, Berta N.
AU  - Quartuccio, Suzanne M.
AU  - Schindler, Karen
DO  - 10.17912/micropub.biology.000491
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000491/
AB  - The Aurora protein kinases (AURK) are essential regulators of chromosome segregation in mitotic and meiotic cell divisions (Carmena and Earnshaw 2003). Unlike mitotically dividing cells which express two AURKs (AURKA and AURKB), mammalian oocytes, which undergo meiosis, express three AURK homologs: AURKA/B/C (Brown et al. 2004; Nguyen et al. 2018). Because AURKC shares high sequence homology with AURKB, standard approaches such as RNAi knockdown (Chen et al. 2005; Sharif et al. 2010) and small molecule inhibition (Chen et al. 2005; Lane et al. 2010; Sharif et al. 2010; Shuda et al. 2009; Swain et al. 2008) to understand their roles do not provide the specificity required to assess non-overlapping functions. Furthermore, these kinases can compensate for one another in oocytes from mouse knockout strains (Balboula and Schindler 2014; Fernandez-Miranda et al. 2011; Nguyen et al. 2018; Schindler et al. 2012), making phenotype assessments less clear. Therefore, alternative strategies to provide specificity are needed.
PY  - 2021
JO  - microPublication Biology
ER  -