TY  - GEN
T1  - Whole Genome Sequencing identifies reciprocal translocation hT2(I;III) breakpoints.
AU  - Flibotte, Stephane
AU  - Edgley, Mark
AU  - Au, Vinci
AU  - Moerman, Donald G
DO  - 10.17912/micropub.biology.000505
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000505/
AB  - The reciprocal translocation hT2 was isolated after gamma irradiation of bli-4(e937)I males followed by crossing to unc-13(e450) I; dpy-18(e364) III hermaphrodites and screening F2 broods for evidence of pseudolinkage (McKim et al., 1993). Subsequent genetic experiments, including variants of hT2 with new morphological mutations induced by mutagenesis, elucidated its genetic structure as a reciprocal translocation and its meiotic pairing and segregation behavior. It is comprised of two half-translocations: hT2(I), which pairs with and disjoins from the normal LG I in hT2 heterozygotes; and hT2(III), which pairs with and disjoins from the normal LG III in heterozygotes. In simple terms, the mutagenesis event that produced hT2 cleaved LG I between unc-101 and unc-59, and LG III between dpy-17 and unc-93; in hT2, the resulting rightmost portion of LG III is translocated to the rightmost portion of LG I to form hT2(I), and the leftmost portion of LG I is translocated to the leftmost portion of LG III to form hT2(III) (see Figure 1).
PY  - 2021
JO  - microPublication Biology
ER  -