TY  - GEN
T1  - Additional Landing Sites for Recombination-Mediated Cassette Exchange in C. elegans
AU  - Nonet, Michael
DO  - 10.17912/micropub.biology.000503
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000503/
AB  - Transgenic animals are powerful tools in the study of basic biological processes using C. elegans. The recent development of recombination-mediated cassette exchange (RMCE) integration approaches in worms provides a relatively rapid efficient method to create single copy transgenes with inserts up to at least 12 kb (Nonet, 2020,Yang et al., 2021). RMCE is similar to MosSCI (Frokjaer-Jensen et al., 2008) in that it depends on integrating at landing sites that have been engineered. In the case of MosSCI, a transposon is excised, and the double stranded break is repaired by a template often using synthesis-dependent strand annealing (SDSA) which is error prone (Frokjaer-Jensen et al., 2008). Similar approaches mediated by cas9 cleavage also have high error rates, approaching 65% in some studies (Au et al., 2019). By contrast, RMCE uses recombination to insert the template into the genome which rarely yield erroneous inserts (Nonet, 2020).
PY  - 2021
JO  - microPublication Biology
ER  -