TY  - GEN
T1  - Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons
AU  - Hsieh, Yi-Wen
AU  - Chuang, Chiou-Fen
DO  - 10.17912/micropub.biology.000530
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000530/
AB  - The UNC-62 homeodomain protein regulates AWC general identity and subsequently plays a cell autonomous role, determined by mosaic analysis, in AWC asymmetry during embryogenesis (Hsieh et al., 2021). An integrated unc-62::GFP fosmid transgene, in which all UNC-62 protein isoforms are tagged with GFP at the C- terminus (Van Nostrand et al., 2013) (Figure 1A), rescued unc-62(lf) mutant phenotypes of AWC general identity, determined by odr-1p::DsRed expression, and AWC asymmetry, determined by str-2p::GFP expression (Hsieh et al., 2021). These results suggest that UNC-62::GFP fusion protein expressed from the unc-62::GFP fosmid transgene is functional for AWC development. It has been shown that this integrated unc-62::GFP fosmid transgene is expressed in sensory neurons, touch neurons, interneurons, ventral nerve cord motor neurons, and head motor neurons, but it is not expressed in AWC in late-stage larvae or young-stage adult worms using the multicolor transgene NeuroPAL (Reilly et al., 2020).
PY  - 2022
JO  - microPublication Biology
ER  -