TY  - GEN
T1  - mNG-tagged mls-2 knock-in alleles in C. elegans
AU  - Xiong, Rui
AU  - Hsieh, Yi-Wen
AU  - Chuang, Chiou-Fen
DO  - 10.17912/micropub.biology.000529
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000529/
AB  - The HMX/NKX MLS-2 transcription factor plays a role in the development of the postembryonic mesoderm, CEPsh glia, tube cells of the excretory system, general AWC identity, and AWC asymmetry in C. elegans (Jiang et al., 2005; Yoshimura et al., 2008; Kim et al., 2010; Abdus-Saboor et al., 2012; Hsieh et al., 2021). The expression pattern of MLS-2 protein has been previously examined by immunohistochemical staining with anti-MLS-2 antibodies, transgenes of GFP-tagged MLS-2 (GFP::MLS-2), and mls-2::GFP fosmid reporter lines (Jiang et al., 2005; Yoshimura et al., 2008; Kim et al., 2010; Abdus-Saboor et al., 2012; Walton et al., 2015; Reilly et al., 2020) (Figure 1A). It was shown that GFP::MLS-2 was expressed in the embryonic AWC lineages from automated lineage analysis and was detected transiently in AWC neurons in first-stage larvae (Kim et al., 2010; Abdus-Saboor et al., 2012; Walton et al., 2015). However, we did not detect expression of GFP::MLS-2 transgenes in AWC neurons in late embryos or the first larval stage (Figure 1B).
PY  - 2022
JO  - microPublication Biology
ER  -