TY - GEN T1 - DiI staining of sensory neurons in the entomopathogenic nematode Steinernema hermaphroditum AU - Garg, Pranjal AU - Tan, Chieh-Hsiang AU - Sternberg, Paul W. DO - 10.17912/micropub.biology.000516 UR - http://beta.micropublication.org/journals/biology/micropub-biology-000516/ AB - Entomopathogenic nematodes (EPNs) form species-specific mutualistic symbiotic relationships with their gut bacteria; on the other hand, they are parasitic to insects, which they infect and rapidly kill with the assistance of their symbiotic bacteria (Dillman and Sternberg, 2012). Studies of EPNs can therefore provide useful insight into parasitism and mutualistic symbiosis. Research in this field has been so far limited by the lack of a genetically tractable system on the host side (Cao et al., 2021). S. hermaphroditum was first isolated in Indonesia (Griffin et al., 2001); that isolate was subsequently lost, but the species was recently rediscovered in India (Bhat et al., 2019). It is consistently hermaphroditic, and techniques and methods developed to study hermaphroditic Caenorhabditis elegans are largely applicable (Cao et al., 2021). Together with its bacterial symbiote Xenorhabdus griffiniae it provides a genetically tractable system for the study of symbiosis. PY - 2022 JO - microPublication Biology ER -