TY  - GEN
T1  - Canavanine resistance mutation can1-1 in Schizosaccharomyces pombe is a missense mutation in the ubiquitin ligase adaptor gene any1
AU  - Yang, Yu-Sheng
AU  - Ning, Shao-Kai
AU  - Lyu, Xiao-Hui
AU  - Suo, Fang
AU  - Jia, Guo-Song
AU  - Li, Wen
AU  - Du, Li-Lin
DO  - 10.17912/micropub.biology.000538
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000538/
AB  - Canavanine is a toxic arginine analog first isolated from jack bean (Canavalia ensiformis) (Kitagawa and Yamada 1932). Because the toxicity of canavanine depends on its cellular uptake mediated by plasma membrane arginine transporters, the isolation of canavanine-resistant mutants in model microorganisms yielded pioneering discoveries on arginine transporter genes (Schwartz et al. 1959; Grenson et al. 1966). In the budding yeast Saccharomyces cerevisiae, all canavanine-resistant mutants isolated are loss-of-function mutants of the arginine transporter gene CAN1 (Whelan et al. 1979; Hoffmann 1985). Canavanine-based counter selection of CAN1 has been widely used as an assay for studying mutagenesis and genome instability in S. cerevisiae (Drake 1991; Tishkoff et al. 1997; Hackett et al. 2001; Lang and Murray 2008; Kim et al. 2011; Jiang et al. 2021).
PY  - 2022
JO  - microPublication Biology
ER  -