TY  - GEN
T1  - swt-3 transcripts are expressed in Z1/Z4 during embryogenesis and in the adult spermatheca
AU  - Updike, Dustin
DO  - 10.17912/W2ZT02
UR  - http://beta.micropublication.org/journals/biology/w2zt02/
AB  - Stellaris smFISH probes targeting swt-3, an ortholog of the human solute carrier transporter SLC50A1, are shown in red (Cal Fluor 610), DAPI in blue, and PGL-1::GFP, a marker for P granules, in green.  A) swt-3 transcripts are expressed in Z1/Z4 cells during embryogenesis (arrowheads).  The embryo shows Z1 and Z4 migrating to Z2/Z3 P granule cells (marked with PGL-1::GFP in green).  B) A dissected proximal gonad of a young adult capturing the ovulation of a recently fertilized zygote (arrow) as it passes through the swt-3 expressing spermatheca (red). Proximal oocyte marked with arrowhead.  C) Sperm nuclei (blue) within the swt-3 (red) spermatheca. scale = 20µ The experiment confirms and extends swt-3 enrichment in cell-specific Z1/Z4 mRNA profiling experiment reported by Kroetz & Zarkower 2015.
PY  - 2017
JO  - microPublication Biology
ER  -