TY  - GEN
T1  - SPAS-1 expression in neurons and vulva during L4 stage​
AU  - Brown, George
AU  - El Bejjani, Rachid
DO  - 10.17912/W2P94X
UR  - http://beta.micropublication.org/journals/biology/w2p94x/
AB  - spas-1 is the worm homolog of the human spastic paraplegia gene, spastin. Previous work in C. elegans has shown that SPAS-1 disassembles microtubules in an ATP-dependent manner (Matsushita-Ishiodori et al, 2007). Another study shows that SPAS-1 plays a role in synapse remodeling in motor neurons (Kurup et al, 2015). Here we describe the expression of SPAS-1 in L4 hermaphrodite larvae using the transcriptional reporter, axrEx8[Pspas-1:mCh]: A) Low magnification (10X objective) image of an entire L4 larva. mCherry expression is driven by the spas-1 promoter (sequence provided). Expression is observed in the nerve ring, ventral cord, vulva, and tail ganglia. Expression is also detected in the intestine (asterisks). B) High magnification (100X objective) image of SPAS-1 expression in the developing vulva of an L4 hermaphrodite. C) High magnification (100X objective) image of nerve ring expression. D-F) SPAS-1 expression in the tail ganglia (TG), ventral cord (VC), and mechanosensory neurons (PLM and AVM shown here). Mechanosensory neuron expression is confirmed by overlaying GFP and mCherry fluorescence in axrEx8 [Pspas-1:mCh]; zdIs5 [Pmec-4:GFP + lin-15 (+)]  animals. Arrows point at the following mechanosensory neurons: PLM=Posterior Lateral Microtubule cell, AVM=Anterior Ventral Microtubule cell.
PY  - 2017
JO  - microPublication Biology
ER  -