TY - GEN T1 - Localization of tbg-1 mRNAs and GFP::TBG-1 protein in Early C. elegans Embryos​ AU - DeMeyer, Lauren AU - Song, Mi Hye DO - 10.17912/W2CW8H UR - http://beta.micropublication.org/journals/biology/w2cw8h/ AB - tbg-1 encodes gamma-tubulin, a ubiquitous and highly conserved component of centrosomes in eukaryotic cells (Strome et al, 2001). Using smFISH we determined the localization of tbg-1 transcripts (red). tbg-1transcripts are detected within distinct foci throughout the cytoplasm during both the first (A) and second (B) mitosis. tbg-1 transcripts are not enriched at centrosomes or either blastomere. In contrast, GFP tagged TBG-1proteins (green signal; arrowheads) localize at centrosomes, as previously shown (Strome et al, 2001). Shown are projections from selected focal planes. Bar=10μm. PY - 2017 JO - microPublication Biology ER -