TY  - GEN
T1  - Microfluidic EPG recordings show striking pharyngeal pumping phenotype in a C. elegans Alzheimer’s disease model
AU  - Weeks, Janis C.
AU  - Robinson, Kristin J.
AU  - Wanjeri, Benedicta
AU  - Copenhaver, Philip F.
AU  - Roberts, William M.
DO  - 10.17912/W2WC7M
UR  - http://beta.micropublication.org/journals/biology/w2wc7m/
AB  - Strain CL4176 accumulates human amyloid-β1-42 in body wall muscles after animals are shifted from 15 to 25 oC, causing paralysis (Link et al. 2003). We temperature-shifted worms at early L4 and quantified pharyngeal pumping at 48 h using a microfluidic electropharyngeogram (EPG) recording platform (Lockery et al. 2012; Weeks et al. 2016). Recordings were made in M9 buffer with 10 mM serotonin and analyzed by automated pump-recognition software. At 48 h, control CL802 worms showed normal pumping activity (A) whereas the mean pump frequency in CL4176 worms was significantly decreased (A; CL802, 4.14 ± 0.11 Hz; CL4176, 2.98 ± 0.18 Hz; mean ± S.E.M.; P <10-5, 2-tailed Student’s t-test; 4 independent replicates). The decreased pump frequency was not a uniform slow-down but instead resulted from a striking increase in the probability of long inter-pump intervals (the time between successive pumps; B, mean ± S.E.M. shown by lines and shading). As seen in B, the normal modal pump frequency of ~4 Hz was still present in CL4176, but the probability of interruptions lasting several seconds was greatly increased.
PY  - 2016
JO  - microPublication Biology
ER  -