TY  - GEN
T1  - xbp-1 mRNA splicing is attenuated under prolonged exposure to ER stress
AU  - Tsialikas, Jennifer
AU  - Argon, Yair
DO  - 10.17912/W2707X
UR  - http://beta.micropublication.org/journals/biology/w2707x/
AB  - IRE-1 is an endoplasmic reticulum (ER) membrane-bound protein that mediates the unfolded protein response (UPR) when cells are under ER stress. Once activated, IRE-1 cleaves an intron from the xbp-1 mRNA (Calfon et al.2002). This yields an active XBP-1 transcription factor, which activates transcription of ER chaperones and other genes involved in protein biosynthesis. Splicing of the xbp-1 intron can be measured by a shift in size in an agarose gel (Shen et al. 2001). Previous studies in mammalian cells showed that there is a sharp increase in Xbp-1 splicing following exposure to ER stress, but that this increase in splicing is attenuated under prolonged exposure to stress (Lin et al. 2007; Eletto et al. 2014). We asked whether this attenuation of xbp-1 splicing is conserved in C. elegans.
PY  - 2017
JO  - microPublication Biology
ER  -