TY  - GEN
T1  - Dominant-negative VPS-4 disrupts ODR-10::GFP distribution but has limited effects on chemotaxis
AU  - Zocher, Ellen
AU  - Ruth, Nelson
AU  - Dahlberg, Caroline
DO  - 10.17912/gnyw-v322
UR  - http://beta.micropublication.org/journals/biology/gnyw-v322/
AB  - Accumulation of ODR-10 depends on the sex and fed state of C. elegans (Ryan et al. 2014), though the precise mechanism of its regulation is not known. We tested whether the ESCRT pathway is required to maintain normal accumulation and distribution of ODR-10::GFP in AWA neurons under well-fed and starved conditions. The conserved AAA ATPase VPS-4 is required as the final step of the ESCRT pathway, to dissociate the machinery as cargo is moved from endosomes to multivesicular bodies (Babst et al. 2011, Kim et al. 2011). Mutation of Glutamate 219 to Glutamine (E219Q) in VPS-4 results in a dominant negative enzyme (VPS-4 DN). VPS-4 DN disrupts normal accumulation of the glutamate receptor, GLR-1, in a cell autonomous manner (Chun et al. 2008).
PY  - 2018
JO  - microPublication Biology
ER  -