TY  - GEN
T1  - Establishment of a CRISPR/Cas9-based strategy for inducible protein dimerization
AU  - Zielich, Jeffrey
AU  - Mangal, Sriyash
AU  - Zanin, Esther
AU  - Lambie, Eric J
DO  - 10.17912/W2208R
UR  - http://beta.micropublication.org/journals/biology/w2208r/
AB  - Induced protein dimerization is a useful tool to study protein function. A well-established method takes advantage of the binding between the FKBP12 protein (FK506 binding protein 12 kDa) and the FRB domain of the mTOR kinase upon interaction with rapamycin (Putyrski and Schultz 2012). Recently we established a rapamycin-inducible dimerization system for the germ line and early embryos of C. elegans (Mangal et al. 2018). We demonstrated the translocation of mCherry::FKBP12 to the plasma membrane via rapamycin induced binding to FRB::GFP::PH (anchored to the plasma membrane). In order to study the function of a native protein upon rapamycin induced translocation it would be advantageous to tag the genomic region of the gene of interest (GOI) with mCherry::fkbp2 to ensure that the fusion protein is controlled by its native environment.
PY  - 2018
JO  - microPublication Biology
ER  -