TY  - GEN
T1  - SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette
AU  - Dickinson, Daniel J
AU  - Slabodnick, Mark M
AU  - Chen, Alicia H
AU  - Goldstein, Bob
DO  - 10.17912/W2KT0N
UR  - http://beta.micropublication.org/journals/biology/w2kt0n/
AB  - The advent of CRISPR/Cas9 technology in C. elegans has enabled an unprecedented level of control over this organism’s genome, which is facilitating research in a wide variety of fields. Several different experimental approaches exist for CRISPR in this organism (Dickinson and Goldstein 2016). One particularly powerful approach uses a drug selection with a Self-Excising Cassette (SEC) to isolate the desired genetically-modified animals following Cas9-triggered homologous recombination (Dickinson et al. 2015). The most labor-intensive step in this procedure is the construction of a plasmid-based repair template for homologous recombination.
PY  - 2018
JO  - microPublication Biology
ER  -