TY  - GEN
T1  - The mapping of Drosophila melanogaster mutant A.4.4
AU  - Bieser, Kayla L
AU  - Stamm, Joyce
AU  - Aldo, Ayala A
AU  - Bhaskara, Suneil
AU  - Clairborne, Makayla
AU  - Coronel Gómez, Joselyn N
AU  - Dean, Ron
AU  - Dowell, Aaron
AU  - Dowell, Evan
AU  - Eissa, Mathew
AU  - Fawaz, Ahmad A
AU  - Fouad-Meshriky, Michael M
AU  - Godoy, Dustin
AU  - Gonzalez, Krista
AU  - Hachem, Malak K
AU  - Hammoud, Malak F
AU  - Huffman, Anthony
AU  - Ingram, Hunter
AU  - Jackman, Alex B
AU  - Karki, Bibek
AU  - Khalil, Natalia
AU  - Khalil, Houda
AU  - Ha, Tran Khanh
AU  - Kharel, Arjun
AU  - Kobylarz, Izabell
AU  - Lomprey, Hunter
AU  - Lonnberg, Adam
AU  - Mahbuba, Safa
AU  - Massarani, Hend
AU  - Minster, Madeline
AU  - Molina, Krystina
AU  - Molitor, Lynette
AU  - Murray, Taylor
AU  - Patel, Payal M
AU  - Pechulis, Sydney
AU  - Raja, Architha
AU  - Rastegari, Gladys
AU  - Reeves, Skylar
AU  - Sabu, Niveda
AU  - Salazar, Rafael
AU  - Schulert, Devan
AU  - Senopole, Matthew D
AU  - Sportiello, Kristen
AU  - Torres, Claudia
AU  - Villalobos, Jade
AU  - Wu, Joseph
AU  - Zeigler, Stacy
AU  - Kagey, Jacob D
DO  - 10.17912/micropub.biology.000069
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000069/
AB  - A novel Drosophila melanogaster mutant A.4.4 was isolated from a conditional Flp/FRT mosaic eye screen in the context of blocked apoptosis (Kagey et al., 2012). The ;FRT42D, Dark82 chromosome was used as a starting point for the EMS mutagenesis screen to screen to screen for mutations that conferred a growth advantage in the environment of blocked apoptosis via the homozygous Dark82 allele (Akdemir et al., 2006). Mutants were screened for over-representation of mutant tissue (pigmented) as compared to the Dark82 mosaic control (Figure 1A).  The mutant mosaic phenotype generated by the cross FRT42D Dark82 A.4.4 X Ey>Flp; FRT42D resulted in mosaic eyes with a slight increase in the red:white ratio (approximately 70:30) as compared to FRT42D Dark82 control eyes (approximately 60:40). Ratios were estimated from observation of multiple mosaic eyes for each genotype. In addition to the increase in mutant tissue, the mosaic A.4.4 eye was observed with a consistent clone/patch of wild type (unpigmented) tissue at the dorsal peak of the eye (Figure 1A, arrow denotes observed region lacking mutant tissue). Whether this mutant phenotype is dependent upon this block in apoptosis is unknown at this time, however other mutant phenotypes in this screen have demonstrated a dependence upon a block in cell death (Kagey et al., 2012).
PY  - 2018
JO  - microPublication Biology
ER  -