TY  - GEN
T1  - FKH-6 is expressed in male gonadal cells during L3 larval development
AU  - Crosby, Aaron
AU  - Kroetz, Mary B
DO  - 10.17912/micropub.biology.000079
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000079/
AB  - FKH-6 is a forkhead transcriptional regulator that is necessary for proper gonadal development in C. elegans. A previous expression study using a transcriptional reporter showed that the fkh-6 transcript was initially expressed in the gonad from mid-L1 larval stage until around the L1/L2 molt in both sexes (Chang et al. 2004). Transcriptional reporters and partial gene fusions indicated that FKH-6 was also expressed from the L3 larval stage through adult in the hermaphrodite gonad in the spermatheca and sheath cells (Hope et al.2003, Chang et al. 2004). In this current study, the endogenous locus for fkh-6 was C-terminally fused with GFP using CRISPR-Cas. In addition to recapitulating previously reported expression patterns, this new construct revealed expression of FKH-6::GFP in 2-4 male gonadal cells in the L3 larval stage. This is the first report of FKH-6 expression in males after the L1/L2 molt. The expression of FKH-6::GFP in the gonad of the L3 male is reproducible but short-lived, and coincides with the period when the proximal region of the migrating male gonad is extending past the distal tip of the gonad. This work shows that FKH-6 is expressed in both sexes during the early mitotic period of the gonad in L1 as well as the late mitotic period of the gonad in L3.
PY  - 2018
JO  - microPublication Biology
ER  -