TY  - GEN
T1  - A CRISPR/Cas9-generated cdc-7 loss of function mutation does not cause temperature-dependent fertility defects
AU  - Currey, Heather
AU  - Liachko, Nicole
DO  - 10.17912/micropub.biology.000085
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000085/
AB  - CDC7 regulates both DNA replication initiation and checkpoint-regulated progression of the cell cycle during the G1/S phase, contributes to DNA recombination and damage repair, and is an essential gene in many species (Yamada et al. 2014).  In C. elegans, there has been a single characterized deletion allele available, tm4391, impacting the 8-exon coding sequence of cdc-7.  tm4391 is missing 315 bp including part of exon 2, all of exon 3, and part of exon 4, and resulting in a downstream frame shift.  cdc-7(tm4391) worms are temperature sensitive sterile, with worse fertility at higher temperatures (Figure 1B).  We generated a novel deletion allele knu709, which is an approximately 2200 bp deletion spanning 150 bp upstream of exon 1 to about 150 bp after exon 8 and removing the entire cdc-7coding sequence.  We tested knu709 for temperature sensitive sterility.  Surprisingly, this strain is not temperature sensitive sterile (Figure 1C), indicating that the fertility defects of tm4391 may be due to either a closely linked mutation in another gene or an unexpected gain-of-function or partial loss-of-function phenotype of cdc-7(tm4391) not recapitulated by a true loss-of-function mutation.
PY  - 2019
JO  - microPublication Biology
ER  -