TY  - GEN
T1  - Genetic mapping of EgfrL.3.1 in Drosophila melanogaster
AU  - Stamm, Joyce
AU  - Joshi, Gnanda S
AU  - Anderson, MA
AU  - Bussing, Katie
AU  - Houchin, Colton
AU  - Elinsky, Amber C
AU  - Flyte, Jacob T
AU  - Husseini, Nadine
AU  - Jarosz, Dominika
AU  - Johnson, Chelsea L
AU  - Johnson, Abby F
AU  - Jones, Christina E
AU  - Kooner, Taj P
AU  - Myhre, Daniel
AU  - Rafaill, Thomas N
AU  - Sayed, Sarah
AU  - Swan, Kirby W
AU  - Toma, Jonathan
AU  - Kagey, Jacob D
DO  - 10.17912/micropub.biology.000098
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000098/
AB  - An EMS screen was conducted utilizing the Flp/FRT system to identify mutations that caused an array of phenotypic alterations in the size of the eye including the ratio of mutant to wild type tissue (red over white) or the developmental patterning of the mosaic eye. This screen was done in the genetic background of blocked apoptosis in the homozygous mutant cells to identify conditional regulators of cell growth and eye development (Kagey et al., 2012). The block in apoptosis in the mosaic mutant tissue was achieved by using a FRT42D Dark82 chromosome as a starting point for the EMS mutagenesis (Akdemir et al., 2006). The Dark82 allele was generated by an imprecise excision of the P{lacW}ArkCD4, this allele retains the w+mC (Akdemir et al., 2006) One of the mutants identified was L.3.1 which generated a small rough eye mosaic phenotype, with a smaller percentage of pigmented tissue than the FRT42D, Dark82 control (Figure 1A). The Dark82 mosaic eye is approximately 60% pigmented tissue, while the Dark82 L.3.1 mosaic eye was smaller overall and approximately 50% mutant tissue (w+mC). The ‘rough eye’ phenotype indicates a disruption in the ommatidial organization. In both images the pigmented (w+mC) tissue is homozygous mutant and the unpigmented tissue is homozygous wild type.
PY  - 2019
JO  - microPublication Biology
ER  -