TY  - GEN
T1  - Caenorhabditis elegans egg-laying and brood-size changes upon exposure to Serratia marcescens and Staphylococcus epidermidis are independent of DBL-1 signaling
AU  - Madhu, Bhoomi J
AU  - Salazar, Aileen E
AU  - Gumienny, Tina L
DO  - 10.17912/2r51-b476
UR  - http://beta.micropublication.org/journals/biology/2r51-b476/
AB  - Caenorhabditis elegans naturally thrives in a soil environment where they feed on bacteria and are in constant association with a diverse range of microbes (Barker et al. 1994). C. elegans egg laying is delayed or reduced when animals are infected with Burkholderia pseudomallei, Burkholderia thailandensis, Staphylococcus aureus, and Serratia marcescens (Mallo et al. 2002; O’Quinn et al. 2002; Irazoqui et al. 2010). These changes in egg laying may be a protective response to pathogenic bacteria. Mutants of TGF-β-like DBL-1 signaling pathway also display reduced brood size (Luo et al. 2009; Roberts et al. 2010).  While the peak of egg-laying activity seen in normal animals between days two and four is depressed in dbl-1 pathway mutants, the reproductive span of these dbl-1 pathway mutants is increased to up to 13 days (Luo et al. 2009). To determine if the egg-laying observed during infection is DBL-1 pathway-dependent, we tested the effect of the DBL-1 signaling pathway on egg laying when C. elegans were fed on representative Gram-negative (S. marcescens) and Gram-positive bacteria (Staphylococcus epidermidis).
PY  - 2019
JO  - microPublication Biology
ER  -