TY  - GEN
T1  - UNC-108/RAB-2 is required for C. elegans stress-induced sleep
AU  - Robinson, Bryan
AU  - Van Buskirk, Cheryl
DO  - 10.17912/micropub.biology.000112
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000112/
AB  - In a genetic screen for mutants defective in stress-induced sleep (SIS) we isolated csn2, an allele of the UNC-108/RAB-2 GTPase. The point mutation in unc-108(csn2) is identical to that in the previously characterized loss-of-function allele unc-108(n3263), substituting a glutamine in place of a glycine that is conserved among Ras superfamily members (Mangahas et al., 2008). Similar to other unc-108(lf) mutants, csn2 animals move slowly (not shown). Here we show that unc-108(csn2) as well as previously characterized unc-108 alleles are SIS-defective (Panels A, B). While the majority of wild-type N2 animals cease head movement, locomotion and pharyngeal pumping following exposure to damaging conditions, unc-108(lf) animals retain all of these activities. This coordinated impairment of sleep-associated behaviors argues against a role for UNC-108 downstream of the SIS-promoting ALA neuron, which acts via the collective action of neuropeptides with overlapping but distinct effects on the sub-behaviors of sleep (Nath et al., 2016).
PY  - 2019
JO  - microPublication Biology
ER  -