TY  - GEN
T1  - The daf-2 insulin receptor functions in C. elegans embryo elongation
AU  - Suresh, Annu
AU  - Wightman, Bruce
DO  - 10.17912/micropub.biology.000117
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000117/
AB  - The daf-2 gene codes for the singular insulin-like growth factor receptor in C. elegans, acting as a control point in multiple developmental and physiological pathways that depend on the integrated response to at least forty different insulin-like peptides (Murphy and Hu, 2013). The functions of daf-2 in dauer formation, longevity, germ-line, and early larval arrest have been extensively studied. A strong daf-2 allele, e979, is homozygous viable at 15oC, despite a partially penetrant embryonic lethal phenotype at 25oC (Gems et al., 1998). The presumed null allele, m65, does not produce fertile homozygous adults due to 100% dauer arrest, and does not display embryonic lethality due to maternal rescue from heterozygous mothers. While several daf-2 alleles display low penetrance embryonic lethality at 25oC, the e979 allele displays the most penetrant embryonic arrest phenotype (Gems et al., 1998; Patel et al., 2008), making it the best choice to investigate the embryonic requirement for insulin signaling. The e979 mutation causes a C146Y substitution that is thought to interrupt an existing disulfide bond interaction with C181, destabilizing the typical fold of the insulin receptor, and impairing the L1 domain’s function in ligand binding (Patel et al., 2008).
PY  - 2020
JO  - microPublication Biology
ER  -