TY  - GEN
T1  - Genetic mapping of shnE.3.2 in Drosophila melanogaster
AU  - Bieser, Kayla
AU  - Sanford, Jamie Siders
AU  - Saville, Ken
AU  - Arreola, Katherine F
AU  - Ayres, Zachary T
AU  - Basulto, David
AU  - Benito, Serena
AU  - Breen, Christopher J
AU  - Brix, Julian A
AU  - Brown, Nicole
AU  - Burton, Krissa K
AU  - Chadwick, Taree M
AU  - Chen, Matthew
AU  - Chu, Katherine
AU  - Corbett, Beverly L
AU  - Dill, Zerrick
AU  - Faughender, Meghan A
AU  - Hickey, Ashlynn D
AU  - Julia, Joshua S
AU  - Kelty, Shannon S
AU  - Jobs, Briggette BK
AU  - Krason, Bryce A
AU  - Lam, Brian
AU  - McCullough, Colin L
AU  - McEwen, Bryanna R
AU  - McKenzie, Julian L
AU  - McQuinn, Kayla R
AU  - Moritz, Chloe M
AU  - Myers, Kristina E
AU  - Naugle, Elizabeth M
AU  - Nutter, Ashley M
AU  - O'Conke, Danielle Q
AU  - O'Grondik, Megan T
AU  - Patel, Kriya B
AU  - Rudowski, Sydney M
AU  - Sberna, Emma N
AU  - Stall, Gunner M
AU  - Steiner, Tad L
AU  - Tanriverdi, Eda
AU  - Torres Patarroyo, Natalie
AU  - Traster, Virginia L
AU  - Tsai, Leo P
AU  - Valenti, Andrew J
AU  - Villegas, Mariela M
AU  - Voors, Samantha M
AU  - Watson, Kierra K
AU  - Wright, Megan E
AU  - Kagey, Jacob D
DO  - 10.17912/micropub.biology.000118
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000118/
AB  - An EMS screen was conducted utilizing the Flp/FRT system to identify mutations that lead to phenotypic alterations in the size of the eye, the ratio of mutant to wild type tissue (red over white), or the developmental patterning of the mosaic eye. This screen was completed in the genetic background of blocked apoptosis in the homozygous mutant cells to identify conditional regulators of cell growth and eye development (Kagey et al., 2012). The block in apoptosis in the mosaic mutant tissue was achieved by using the FRT42D Dark82chromosome, which retains the w+mC(pigmentation), as a starting point for the EMS mutagenesis (Akdemir et al., 2006). One of the mutants identified was mutant E.3.2. The mutant mosaic phenotype, generated from the cross FRT42D Dark82 E.3.2 XEy>Flp; FRT42D, resulted in a range of phenotypes. This included, gross eye pattern disruption, abnormal shape, and antennal overgrowth (see bottom image) when compared to the FRT42D Dark82 mosaic controls (Figure 1A). The control mosaic phenotype had a characteristic 60:40 red:white ratio as compared to the mutant mosaic phenotype of approximately 30:70 red:white ratio. This indicates a reduction in mutant tissue, but included abnormal cranial, eye, and antennal development (Figure 1A).
PY  - 2019
JO  - microPublication Biology
ER  -