TY  - GEN
T1  - Isolation of intragenic suppressor of tsp-15-splicing mutant in Caenorhabditis elegans
AU  - Moribe, Hiroki
AU  - Mekada, Eisuke
DO  - 10.17912/micropub.biology.000126
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000126/
AB  - TSP-15 is one of the 21 tetraspanins in Caenorhabditis elegans and is essential for exoskeletal (cuticle) development. A reduction in the function of TSP-15 results in a dumpy (Dpy) and/or blistered (Bli) phenotype (Moribe et al. 2004), and TSP-15 null mutants exhibit embryonic lethality (Moribe et al. 2012). sv15 is a hypomorphic allele of tsp-15, featuring a splicing error mutation in tsp-15’s fourth intron. The mutation results in the immediate production of a stop codon (Fig. 1A), and the resulting truncated form of TSP-15 protein has no function. sv15 mutants are viable despite severe cuticle deficiencies since some correctly spliced transcripts are produced (Moribe et al. 2004).
PY  - 2019
JO  - microPublication Biology
ER  -